Cardiolipin content and protoplast fusion efficiency in B. subtilis
نویسندگان
چکیده
منابع مشابه
Electron microscopic study of Bacillus subtilis protoplast fusion.
When protoplasts derived from sporulating cells of Bacillus subtilis were fused by exposure to polyethylene glycol (PEG) and fixed immediately thereafter, protoplasts with two enclosed prespores could be seen by electron microscope. The number of fusion events was greatly increased, and multiply fused protoplasts appeared, when the PEG-treated suspension was diluted in hypertonic broth and rein...
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Brassica coenospecies has been bestowed with a large collection of wild and weedy relatives, which could act as donors of genes controlling agronomically important traits like disease resistance, yield attributes, modified fatty acid composition in the seed etc. Additionally, the cytoplasm of these species could be the sources for developing alloplasmic male sterility systems that are important...
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Fusions of Bacillus subtilis protoplasts from two genetically marked strains produce noncomplementing heterodiploid bacteria. These noncomplementing diploids (Ncds) carry both parental chromosomes, but only one is expressed. Fusion products of strains polymorphic for NotI restriction sites provide new physical evidence to support the conclusion that Ncds are not an artifact of cross feeding or ...
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The subjects of this article are protoplast isolations and protoplast fusions, in particular their history, a review of factors influencing the protoplasts isolation and fusion, selection of hybrid plants and utilization of somatic hybrids in plant breeding. Somatic hybridization through protoplast fusion can overcome sexual incompatibility among plant species or genera; transfer genes of resis...
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Intraspecific hybrid production from the polyethylene glycol induced fusion of yeast protoplasts was greatly increased when calcium propionate was included as the source of the requisite Ca2+. The use of calcium propionate, as opposed to the more commonly employed calcium chloride, resulted in substantially greater yields of hybrids from intraspecific fusions of protoplasts of Saccharomyces cer...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1983
ISSN: 0378-1097
DOI: 10.1016/0378-1097(83)90376-2